2nd World Congress on Animal Science & Veterinary Medicine

November 03-04, 2025       Grand Mercure Bangkok Atrium, Thailand

Ms. Ananya Suresh

Ms. Ananya Suresh

University of Georgia
USA

Abstract Title: FITC-D Permeability Assay to Analyze Loss in Gut Integrity during Coccidial Infections in Broiler Birds

Biography:

Ms. Ananya Suresh is a Grade 11 student from APL Global School, Chennai, with a strong academic interest in life sciences and has participated in science fairs and academic workshops demonstrating a commitment to problem solving and scientific enquiry. Beyond academics, Ananya has been shortlisted in Global Essay Competition of John Locke Institute, UK and is a gold medalist in the Cambridge Science Competition (2024). She aspires to pursue higher education in the health, biomedical or data science field, with the goal of driving innovation and positive change on a global scale.

Research Interest:

Poultry coccidiosis is characterized by damage to the epithelial lining of the intestines, leading to increased paracellular passage of non-absorbable markers, such as fluorescein isothiocyanate-dextran (FITC-Dextran), into the bloodstream and eventual impaired production performance. The objective of this study is to measure the coccidiosis-induced decrease in gut integrity and production performances in broilers. A total of 216 birds were randomly divided into two treatment groups, Control and Coccidia, in six replications (n=6) with 18 birds/pen. On d14, birds were challenged orally with Eimeria acervulina, E. maxima and E. tenella at 62,500, 12,500 and 12,500 oocysts/bird. Weekly body weight gain (BWG), feed consumption (FC) and feed conversion ratio (FCR) were measured. On d 14, 21, and 28, gut integrity was measured through fluorescein isothiocyanate-dextran (FITC-Dextran) permeability assay. Data were analyzed by ANOVA (P < 0.05). Coccidiosis infection decreased (P < 0.05) the BWG on d 14-21 by 15% and on d 21-28 by 26%. Coccidiosis infection decreased (P < 0.05) the FC on d 21-28 by 20%. Coccidiosis infection decreased (P < 0.05) the FCR on d 14-21 by 14%. Coccidiosis infection increased (P < 0.05) the gut permeability on d 21 by 63% and on d 28 by 25%. It can be concluded that mixed coccidiosis infection decreased the production performance and resulted in loss of gut integrity until 14 days post-infection. FITC-Dextran analysis can be used as an assay for measuring loss in gut integrity in broiler birds.